Biotech methods for making lab-grown poultry meat using stem cell reprogramming and gene editing

The biofabricated poultry product is compositionally optimized, free of antibiotic residues, and exhibits a significantly reduced environmental footprint compared to conventional poultry production.

• Combining induced pluripotent stem cell reprogramming and CRISPR-Cas9 genome editing may enhance the myogenic potential and growth efficiency of avian flesh.
• Ectopic expression of Yamanaka factors is used to reprogram primary somatic cells from Gallus gallus into induced pluripotent stem cells.
• Cultivation of iPSCs in a xeno-free bioreactor allows for the development of myoblasts and mature myotubes.
• Three-dimensional tissue biofabrication is achieved by integrating biomaterial scaffolds with perfusion bioreactor systems.
• The engineering platforms enable precise control over microenvironmental factors such as oxygenation and nutrient delivery.

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