Effect of cadmium on 24-hour pattern in expression of redox enzyme and clock genes in rat medial basal hypothalamus

Jan 29, 2010Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine

Cadmium's impact on daily patterns of antioxidant and biological clock gene activity in a key rat brain region

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Abstract

A low dose of cadmium (7.5 microg/day) resulted in significant changes in gene expression patterns in the medial basal hypothalamus of rats.

  • Cadmium exposure led to a disruption in the 24-hour expression patterns of several redox pathway enzymes in the brain.
  • Mean mRNA levels for heme oxygenase-2, manganese superoxide dismutase, and catalase increased following cadmium intake.
  • Nitric oxide synthase-2 levels decreased after exposure to cadmium.
  • The circadian expression patterns of clock genes, such as Bmal1, Per1, Per2, and Cry2, were significantly altered by cadmium.
  • Cadmium exposure induced significant 24-hour variations in the expression of the Clock gene.

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