BACKGROUND: Caffeine's ergogenic potential in vertical jumping remains contested, particularly at dosages compliant with collegiate athletic regulations. The NCAA enforces a caffeine urinary threshold equivalent to ~5 mg/kg body mass, yet evidence supporting its efficacy in enhancing explosive performance among trained athletes is inconsistent. This study examined whether acute caffeine ingestion at this threshold improves squat jump (SJ) and countermovement jump (CMJ) performance in NCAA Division II student-athletes, while exploring sex-specific responses and trial-to-trial variability.
METHODS: Forty NCAA Division II athletes (18 females, 22 males; 21.3 ± 2.4 years) participated in a single-blind, randomized, crossover trial. Participants ingested 5 mg/kg caffeine or placebo, followed by SJ and CMJ testing on a force platform 60 minutes post-consumption. Three trials per jump type were retained to preserve intra-individual variance. Linear mixed-effects models (LMMs) assessed fixed effects of condition, sex, and trials, with random intercepts and slopes accounting for individual variability. A priori power analyses guided sample size determination, targeting sensitivity to detect small-to-medium effects (Cohen's f ≥ 0.2).
RESULTS: Caffeine elicited trivial, non-significant differences in SJ (β = -0.24 cm, = 0.646) and CMJ (β = -0.71 cm, = 0.183) heights compared to placebo. Males outperformed females in both SJ (Δ = 9.50 cm, < 0.001) and CMJ (Δ = 11.30 cm, < 0.001), though no condition-by-sex interactions emerged. Trial effects were observed, with SJ improving 2.31 cm ( < 0.001) and CMJ 1.05 cm ( = 0.014) from first to second attempts, suggesting warm-up or neuromuscular potentiation. High intraclass correlation coefficients (ICC = 0.76-0.85) underscored substantial interindividual variability. Models demonstrated robust fit (conditional R = 0.86-0.92), with sufficient power to detect medium-to-large effects (f ≥ 0.25). p p p p p p2
CONCLUSIONS: A 5 mg/kg caffeine dose, compliant with NCAA thresholds, did not enhance vertical jump performance in habituated collegiate athletes, challenging prior assertions of its universal ergogenicity for ballistic tasks. While sex differences in baseline performance persisted, caffeine did not modulate these disparities, implicating neuromuscular and anthropometric factors as primary determinants. The absence of ergogenic effects aligns with NCAA safety-focused policies but highlights the need for personalized dosing strategies and research into higher doses, genetic variability, and hormonal influences. Future studies should integrate biomechanical metrics and controlled hormonal assessments to elucidate caffeine's nuanced role in anaerobic power sports.
