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Capillary electrophoresis methods for determining the IVT mRNA critical quality attributes of size and purity
Using capillary electrophoresis to measure the size and purity of lab-made mRNA
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Abstract
IVT mRNAs can range from less than 100 nt to longer than 9,000 nt.
- Quality control is essential for producing IVT mRNA to ensure the correct length and absence of degradation.
- Capillary gel electrophoresis offers high-resolution separation for assessing the purity of IVT mRNA.
- The method allows differentiation between intact mRNA and truncated or degraded forms.
- Specialized gels can analyze poly(A) tails, enabling the characterization of multiple Critical Quality Attributes.
- Purity assessment methods can be applied to IVT mRNAs measuring either 6,000 or 9,000 nt.
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