The chromatin remodeling and mRNA splicing functions of the Brahma (SWI/SNF) complex are mediated by the SNR1/SNF5 regulatory subunit

Apr 3, 2012Nucleic acids research

The SNR1/SNF5 subunit controls DNA packaging and mRNA processing functions of the Brahma (SWI/SNF) complex

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Abstract

Incorporation of a loss of function SNR1 subunit led to alterations in RNA polymerase elongation and pre-mRNA splicing regulation.

  • Nucleosome remodeling by the is essential for regulated gene expression.
  • The Drosophila Brahma (Brm) SWI/SNF complex responds to ecdysone hormone signaling during development.
  • Loss of chromatin remodeling function is linked to developmental abnormalities and aggressive cancers.
  • plays a unique role in regulating target gene expression by restricting BRM-dependent nucleosome remodeling.
  • Changes in chromatin accessibility of hormone-regulated genes were observed with SNR1 dysfunction.

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Key numbers

79-fold
Increase in spliced product
Measured in developmental stages where transcripts are detected.
6-fold
Reduction in 3′ transcript
Observed in early pupae with RNAi depletion of NELF.

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