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Abstract
Recent advances in genome editing allow for the precise generation of defined chromosomal abnormalities in human cells.
- Chromosomal aberrations, such as aneuploidies and structural rearrangements, are important factors in cancer and genetic disorders.
- Current techniques include CRISPR-based chromosome elimination, KaryoCreate, prime editing, programmable recombinases, and RNA-guided bridge recombinases.
- These methods enable modeling of both numerical and structural chromosomal changes.
- Strategies for creating complex chromosomal rearrangements are also discussed.
- These technologies may enhance the understanding of the early genomic events that contribute to cancer and genetic diseases.
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