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Circadian clock control of interactions between eIF2α kinase CPC-3 and GCN1 with ribosomes regulates rhythmic translation initiation
The body clock controls how two proteins interact with ribosomes to regulate daily patterns of starting protein production
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Abstract
Circadian rhythms in mRNA translation through activity require rhythms in levels.
- GCN2 activity is influenced by uncharged tRNA levels, which activate the kinase to phosphorylate eIF2α during nutrient starvation.
- The circadian clock regulates the interaction of CPC-3 and with ribosomes, essential for maintaining CPC-3 activity rhythms.
- A temperature-sensitive mutant with high uncharged tRNA levels disrupts rhythmic interactions and abolishes CPC-3 activity rhythms.
- Disrupting the interaction between GCN1 and uncharged tRNA affects the rhythmic activity of CPC-3 in the absence of GCN20.
- These findings suggest a mechanism linking circadian rhythms to the regulation of mRNA translation and cellular energy levels.
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