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Association of GCN1-GCN20 regulatory complex with the N-terminus of eIF2alpha kinase GCN2 is required for GCN2 activation
Activation of the stress-sensing kinase GCN2 requires its regulatory complex to bind its starting region
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Abstract
GCN1-GCN20 complex is essential for activating GCN2 by uncharged tRNA.
- The GCN1-GCN20 complex binds to the N-terminus of GCN2, indicating a direct interaction.
- Overexpression of N-terminal segments of GCN2 can hinder its association with GCN1-GCN20, leading to a dominant-negative effect.
- The negative phenotype caused by overexpressing N-terminal GCN2 segments can be reversed by increasing levels of GCN2, GCN1-GCN20, or uncharged tRNA.
- In a strain lacking GCN1, the need for GCN1 is diminished when uncharged tRNA is overexpressed.
- The interaction between GCN1-GCN20 and GCN2 appears to be evolutionarily conserved, as shown by the similar behavior of Drosophila GCN2.
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