Strong daily clock rhythms persist despite constant high levels of mCry1 in rat fibroblast cells

Robust circadian clock oscillation persists even with constitutive over-expression of mCRY1 in rat-1 cells.

• mCRY proteins are critical components that negatively regulate circadian rhythms in mammals.
• The study utilized rat-1 cells engineered to express a luciferase reporter driven by the mPer2 promoter.
• Circadian clock oscillations were maintained despite the continuous accumulation of mCRY1 in the cell nuclei.
• The findings suggest that the presence of mCRY1 alone does not disrupt the circadian feedback loop.

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