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A Role for the Circadian Clock Protein Per1 in the Regulation of the NaCl Co-transporter (NCC) and the with-no-lysine Kinase (WNK) Cascade in Mouse Distal Convoluted Tubule Cells
The circadian clock protein Per1 may regulate salt transport and related signaling proteins in kidney cells
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Abstract
Pharmacological blockade of nuclear Per1 entry resulted in decreased mRNA expression of NCC and WNK1 but increased expression of WNK4 in the renal cortex of mice.
- Per1 regulates sodium transport in the distal convoluted tubule (DCT) by modulating the expression of the NaCl co-transporter (NCC) and with-no-lysine kinases (WNKs).
- Inhibition of Per1 nuclear entry led to decreased NCC protein expression and activity, indicating a functional impact on sodium reabsorption.
- Chromatin immunoprecipitation experiments showed that Per1 interacts with the promoters of NCC, WNK1, and WNK4, suggesting transcriptional regulation.
- Changes in short-lived heterogeneous nuclear RNA indicate that Per1 may affect gene expression dynamics in the DCT.
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