Identification of a Circadian Output Circuit for Rest:Activity Rhythms in Drosophila

Apr 29, 2014Cell

A daily activity control circuit for rest and movement rhythms in fruit flies

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Abstract

Six specific DH44+ pars intercerebralis cells in Drosophila are critical for regulating rest:activity rhythms.

  • Circadian output pathways linking clock cells to behaviors have not been fully characterized before this analysis.
  • The pars intercerebralis (PI) neurons, similar in function to the mammalian hypothalamus, are identified as key components of the circadian output pathway.
  • A polysynaptic circuit connects pacemaker cells to PI neurons, allowing communication between the circadian clock and locomotor activity.
  • Molecular profiling indicates that the molecule DH44, a homolog of corticotropin-releasing factor, is specifically produced by PI neurons.
  • Activation or removal of just six DH44+ PI cells can disrupt normal rest:activity rhythms.

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