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Distinct cis-Acting Elements Mediate Clock, Light, and Developmental Regulation of the Neurospora crassa eas (ccg-2) Gene
Separate DNA regions control daily rhythm, light response, and growth stage effects on a key Neurospora gene
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Abstract
A 45-bp region upstream of the TATA box is critical for the clock regulation of the eas (ccg-2) gene in Neurospora crassa.
- The eas (ccg-2) gene encodes a hydrophobin and is regulated by the circadian clock and light.
- Transcripts of eas (ccg-2) accumulate during asexual development.
- Deletion analyses identified a positive activating clock element (ACE) that is essential for clock regulation.
- A 68-bp sequence containing the ACE was found to be sufficient for conferring clock regulation on the eas (ccg-2) gene.
- Specific DNA-binding factors in N. crassa protein extracts recognize the ACE.
- Separate regions of the eas (ccg-2) promoter are involved in light induction and developmental control but are not required for clock-regulated expression.
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