Advanced science (Weinheim, Baden-Wurttemberg, Germany)Oct 16, 2026

Blocking COMPASS Promoter Activation Makes HDAC-Inhibited Gastric Cancer Keep Genes Turned Off During Recovery

CRISPR Gene Editing Weekly Brief ↗PubMed ↗DOI ↗OA ↗

Updated Oct 17, 2026

Abstract

WDR5 abundance and baseline H3K4me3 levels are associated with sensitivity to combined HDAC and WDR5 WIN-pocket inhibition.

Histone deacetylase inhibitors increase chromatin acetylation but show variable effectiveness in solid tumors.
The study identifies the WDR5 WIN pocket as crucial for maintaining chromatin-associated COMPASS and promoter-proximal H3K4me3 levels.
Disruption of the WDR5 WIN pocket leads to reduced recovery of COMPASS components and lower H3K4me3 at specific promoters.
This disruption results in higher H3K27 acetylation, decreased H3K4me3, and diminished transcriptional output.
Data indicate that HDAC inhibitor response may be influenced by promoter licensing through the WDR5-COMPASS H3K4 methylation axis.

Simplified

Histone deacetylase inhibitors (HDACi) broadly increase chromatin acetylation but have limited and variable activity in solid tumors. To define the epigenetic buffering axis that sustains promoter output during HDAC inhibition in gastric cancer. We integrated pharmacologic profiling across a molecularly diverse gastric cancer panel with an epigenetic-focused pooled CRISPR-Cas9 screening library, domain-resolved genetic perturbation, structure-guided WIN-pocket targeting, CCK8 time-course proliferation assays, and pulse-washout clonogenic recovery assays. Chromatin and transcriptional consequences were interrogated by assessing COMPASS integrity and chromatin association, promoter-proximal H3K4me3 dynamics, data-derived ATAC-seq accessibility, and transcriptional output. WDR5 abundance and baseline H3K4me3 levels were associated with sensitivity to combined HDAC and WDR5 WIN-pocket inhibition. Multiple orthogonal approaches identified the WDR5 WIN pocket as a key interface supporting chromatin-associated COMPASS and promoter-proximal H3K4me3 under the tested conditions. WIN-pocket disruption reduced chromatin recovery of COMPASS-associated components and attenuated H3K4me3 at selected growth-linked promoters. This produced a state with elevated H3K27 acetylation, attenuated H3K4me3, and reduced transcriptional output; data-derived ATAC-enriched regions retained a centered accessibility signal, although the mean peak amplitude was lowest in the combination. HDAC inhibitor response is conditioned by promoter licensing via the WDR5-COMPASS H3K4 methylation axis.

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Blocking COMPASS Promoter Activation Makes HDAC-Inhibited Gastric Cancer Keep Genes Turned Off During Recovery

Abstract

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