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A Computer-Designed Method to Make Circular RNA Without Unwanted Byproducts
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Abstract
A versatile self-circularization strategy for mRNA synthesis achieves robust efficiencies for sequences ranging from dozens to thousands of nucleotides.
- Circular mRNA is associated with increased stability and prolonged protein translation compared to linear mRNA.
- The method utilizes optimized lock-key structures to enhance circularization efficiency, especially for longer RNA sequences.
- No additional purification steps are necessary due to the functional similarities between linear precursor RNAs and circular products.
- The produced circular RNAs demonstrate superior stability and translation efficiency, potentially enabling sustained protein expression.
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