Contractile reactivity of human myometrium in isolated non-pregnant uteri

Sep 13, 2005Human reproduction (Oxford, England)

Muscle contraction responses of the human womb in isolated non-pregnant samples

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Abstract

Continuous treatment with 17beta-estradiol (E2) and oxytocin (OT) led to the most pronounced uterine contractions during a 27-hour perfusion of isolated uteri.

The perfusion model demonstrated feasibility for studying human uterine tissue.
Group D, receiving continuous E2 followed by OT, exhibited the highest myometrial activity.
No significant contractions were observed in the control group without treatment.
The timing of OT administration appears to influence the contractile response of the myometrium.

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BACKGROUND: Perfusion of the isolated uterus has been shown to be a feasible experimental system for studies of the human endometrium and myometrium. Utilizing our established experimental perfusion model we perfused 20 uteri for 27 h and investigated the contractile reactivity of the myometrium in response to 17beta-estradiol (E2) and oxytocin (OT).

METHODS: Uteri of group A (n = 4) were stimulated with OT; group B (n = 4) was treated continuously with E2; group C (n = 4) received both E2 and OT for 27 h; group D (n = 4) was perfused for 27 h with E2 with the addition of OT for the last 3 h of the experiment; group E (n = 4) as control group remained without any treatment. The pressure and duration of uterine contractions were recorded during the entire perfusion period using intramural and endoluminal pressure catheters.

RESULTS: Compared to the other treatment groups and controls, the most effective myometrial activity was achieved in group D during the OT stimulation period. No relevant myometrial activity was detected in the control group.

CONCLUSIONS: Continuous E2 treatment, with the addition of OT for the last 3 h of the 27 h perfusion period, led to the most pronounced uterotonic effects in the presented experimental condition.

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Contractile reactivity of human myometrium in isolated non-pregnant uteri

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