cPRIME allows natural mapping of genetic variant effects on RNA

cPRIME enables the assessment of variant effects at RNA level using prime editing.

• cPRIME allows for the insertion of genetic variants into specific locations in the genome.
• Quantification of variant frequencies is performed at both RNA and genomic DNA levels.
• The method is effective across various karyotypes and genomic locations.
• Synonymous marker edits can influence RNA processing, complicating the interpretation of variant effects.
• cPRIME provides a means to study the post-transcriptional effects of genetic variants within their genomic context.

Simplified