Nucleic acids researchFeb 23, 2026

Using CRISPR-related transposons to design viral delivery tools and precise gene editing

CRISPR Gene Editing Weekly Brief ↗PubMed ↗DOI ↗OA ↗

Updated Feb 26, 2026

Abstract

SHOT 2.0 achieves integration of large DNA cargos (at least 14 kb) into defined loci.

The optimized platform enables customizable bacmid editing in Escherichia coli.
Integration at the ODVe56 locus significantly enhances transgene stability during serial virus passaging.
The system allows for dual-gene insertion and is compatible with the Bac-to-Bac® workflow.
An all-in-one baculovirus was constructed to encode the PE5max prime editor.
efficiencies reached up to 85.6% in HEK293T cells and 37.1% in hard-to-transfect cell types.

Simplified

Key numbers

85.6%

Efficiency in HEK293T Cells

Efficiency of achieved using the SHOT 2.0 platform.

37.1%

Efficiency in Liver Cancer Cells

Efficiency achieved in hard-to-transfect liver cancer cells using the SHOT 2.0 platform.

14 kb

Large DNA Cargo Integration

Minimum size of DNA fragments that can be integrated using SHOT 2.0.

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Funding

Competing interests

None declared.

PubMed

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