Genome biology

BEstimate: a tool to plan and understand CRISPR base editing experiments

CRISPR Gene Editing Weekly Brief ↗PubMed ↗DOI ↗OA ↗

Updated

Abstract

BEstimate is a new computational tool for designing guide RNAs that may enhance targeted DNA mutagenesis.

  • BEstimate systematically identifies target sites for base editor guide RNAs.
  • It predicts both on-target activity and potential off-target effects of designed gRNAs.
  • The tool provides functional, structural, and clinical annotations for installed DNA variants.
  • BEstimate allows customization of gRNA designs for specific variant alleles.
  • It supports the reversion of disease-associated genetic variants.

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Full Text

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Funding

Competing interests

Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: M.J.G. reports research grants from GlaxoSmithKline and Astex Pharmaceuticals. M.J.G. is a founder and advisor at Mosaic Therapeutics. M.A.C. and M.J.G. are co-founders of BASE Rx. C.D. and B.F. declare that they have no competing interests.
PubMed

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