BASELINE: A Gene-Editing Tool for Tracking Cell Lineage in Mammals at Single-Cell Level

BASELINE records over 4300 bits of lineage information, significantly enhancing lineage tracing capabilities in cellular research.

• BASELINE utilizes a specific base editing technique to generate detailed lineage trees at the single-cell level.
• The technology enables the irreversible editing of nucleotides across 50 synthetic target sites integrated into a cell's genome.
• It captures lineage-specific marks across a variety of biologically relevant intervals, showing a 50X increase in data accumulation compared to previous methods.
• Single-cell sequencing demonstrates the ability to recover lineage reconstructions up to 40 cell divisions deep.
• This approach may facilitate lineage-tracing projects in both developmental biology and disease contexts, particularly where conventional methods face limitations.

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