Methods in molecular biology (Clifton, N.J.)

Precise DNA base changes in rice using CRISPR/Cas base editing

CRISPR Gene Editing Weekly Brief ↗PubMed ↗DOI ↗

Updated

Abstract

Base editors enable precise genome editing without inducing DNA double-strand breaks.

  • Base editing is a method that allows for specific base conversions at targeted DNA sites.
  • The effectiveness of base editors is limited by the requirement for a specific sequence known as the protospacer adjacent motif (PAM).
  • The widely used Cas9 from Streptococcus pyogenes requires an NGG sequence for PAM recognition.
  • Engineered and natural variants of Cas9 have been developed to expand the range of usable PAM sequences.
  • SpCas9-NG is a variant that can recognize more flexible PAM sequences, enhancing base substitution capabilities.

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