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Precise Base Substitution Using CRISPR/Cas-Mediated Base Editor in Rice
Precise DNA base changes in rice using CRISPR/Cas base editing
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Abstract
Base editors enable precise genome editing without inducing DNA double-strand breaks.
- Base editing is a method that allows for specific base conversions at targeted DNA sites.
- The effectiveness of base editors is limited by the requirement for a specific sequence known as the protospacer adjacent motif (PAM).
- The widely used Cas9 from Streptococcus pyogenes requires an NGG sequence for PAM recognition.
- Engineered and natural variants of Cas9 have been developed to expand the range of usable PAM sequences.
- SpCas9-NG is a variant that can recognize more flexible PAM sequences, enhancing base substitution capabilities.
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