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Abstract
CRISPR-Cas treatment can cause large unintended deletions, including surrounding chromosomal sequences.
- HIV replication can be effectively inhibited by antiretroviral therapy, but this does not eliminate the virus.
- Lifelong therapy is necessary for HIV-infected individuals to prevent viral rebound.
- Preclinical studies suggest that CRISPR-Cas gene-editing systems may permanently inactivate integrated HIV-1 DNA.
- Inactivation of HIV-1 DNA has been linked to small mutations and excision or inversion of viral DNA fragments.
- Large unintended deletions associated with CRISPR-Cas treatment may pose a safety risk due to the potential for oncogenic transformation.
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