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Abstract
Split CRISPR/Cas systems may achieve significant improvements in sensitivity for femtomolar-level biomarkers.
- Split-activation strategies enhance analytical performance without the need for pre-amplification.
- Two key approaches include split activator-mediated Cas systems for sensitive RNA detection and split crRNA architectures for analyzing multiple nucleic acids.
- These innovations maintain superior single-base discrimination while improving detection capabilities.
- Applications include clinical diagnostics and on-site monitoring, though challenges such as background noise and cost remain.
- Future developments may focus on customized Cas variants and integrated platforms for improved diagnostics.
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