A Self-Activating CRISPR System Using a Switchable Probe for Highly Sensitive DNA Detection

This amplification-free CRISPR/Cas-based diagnostic system demonstrates atto-molar sensitivity for pathogen detection.

• The system uses a structure-switching V-shaped DNA probe with a Cas12a recognition sequence to enhance detection sensitivity.
• It produces a rapid exponential signal while maintaining an ultralow background signal.
• Applications include multiplex detection of distinct papillomavirus strains and point-of-care detection of monkeypox virus infections.
• The approach may facilitate quicker and simpler pathogen detection in clinical and point-of-care settings.

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