Genome medicineSep 18, 2026

Precise analysis of CRISPR-Cas9 gene editing results and mixed cell changes using highly accurate long DNA reads

CRISPR Gene Editing Weekly Brief ↗PubMed ↗DOI ↗OA ↗

Updated Sep 19, 2026

Abstract

Genetic mosaicism is a recognized consequence of CRISPR-Cas9 genome editing.

Characterizing genetic mosaicism involves identifying low-frequency structural variants.
Deep and unbiased sequencing is necessary for a comprehensive analysis of mosaicism.
Accurate single-molecule reads are crucial for effective characterization of target loci.

Simplified

Genetic mosaicism is a well-recognized consequence of CRISPR-Cas9 genome editing, yet its characterization remains challenging, especially when it involves low-frequency structural variants. A comprehensive analysis of mosaicism requires deep and unbiased sequencing of the target loci, with accurate single-molecule reads.

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