Stem cell research

Using gene editing to remove FLG in human stem cells to model skin barrier problems

CRISPR Gene Editing Weekly Brief ↗PubMed ↗DOI ↗

Updated

Abstract

A FLG knockout human induced pluripotent stem cell line was established using CRISPR/Cas12a genome editing.

  • Loss of filaggrin function is linked to impaired skin barrier formation and inflammatory skin diseases.
  • The edited iPSC line showed typical pluripotent stem cell characteristics and maintained the ability to differentiate into all three germ layers.
  • Genomic stability was confirmed through karyotype and copy number variation analyses.
  • The cell line was free of mycoplasma contamination.
  • This model may facilitate research into filaggrin-associated skin biology and related diseases.

Simplified

Full Text

Full text is available at the source.
View Full Text ↗

Funding

Competing interests

Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Andrea Rossi reports financial support was provided by IUF Leibniz Research Institute for Environmental Medicine. ANDREA ROSSI has patent hiPSCore pending to Andrea Rossi. N.A. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
PubMed

What Lands in Your Inbox Each Week:

  • 📚7 fresh studies
  • 📝plain-language summaries
  • ✅direct links to original studies
  • 🏅top journal indicators
  • 📅weekly delivery
  • 🧘‍♂️always free

Recent issues from the crispr gene editing brief

issue #48
CRISPR base editors delivered to rat brains reduced Huntington's protein fragments and improved movement
2 days ago7 studies
issue #47
AI tool uses AlphaFold3 to pinpoint exactly which gene editor residues cause off-target cuts
1 week ago7 studies
issue #46
A new delivery trick pushed prime editing efficiency up to 2.9-fold without extra off-target edits
2 weeks ago7 studies
issue #45
Scientists built humanized antibody mice in 8 weeks instead of over a year
3 weeks ago7 studies
issue #44
Scientists mapped 11,692 gene knockdowns in stem cells — all in one atlas
4 weeks ago7 studies
issue #43
A rat virus enzyme boosted gene editing efficiency by 1.88x at hard-to-edit DNA sites
5 weeks ago7 studies
issue #42
A single dose edited 49% of liver cells in mice — without a virus
6 weeks ago7 studies
issue #41
CRISPR gene therapy eliminates blood transfusions in 91% of β-thalassemia patients
7 weeks ago7 studies
issue #40
90% of CRISPR guide RNA modified with new chemistry maintains full gene editing power
8 weeks ago7 studies
issue #39
AI-designed gene editor cuts off-target mutations by 553-fold
9 weeks ago7 studies
explore the archive
See more issues from the crispr gene editing brief