Using base and prime editors to analyze disease-related genetic changes on a large scale

Base and prime editors enable precise modifications in the genome without introducing double-strand breaks.

• Base and prime editing platforms systematically annotate clinical variants.
• These technologies reveal mechanisms of drug resistance and immune evasion.
• Functional screens using these editors can dissect fundamental biological processes at the level of single nucleotides.
• Challenges and future perspectives for precision editing screens are addressed.
• Causal genotype-to-phenotype mapping is facilitated, potentially redefining genomic variation interpretation.

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