Current abilities and challenges of DNA base editing tools

CRISPR-mediated DNA base editors can induce point mutations with high efficiency without causing DNA double-strand breaks.

• Cytosine base editors (CBEs) and adenine base editors (ABEs) are key types of DNA base editors.
• These tools may correct or disrupt gene expression at specific sites.
• Despite their promise, DNA base editors may have significant shortcomings, including off-target editing.
• Bystander mutations and unintended effects in both DNA and RNA are associated with the use of these editors.
• Current efforts aim to address and reduce the challenges linked to DNA base editing.

Simplified