Dopamine D1 Receptor-Mediated Regulation of Per1, Per2, CLOCK, and BMAL1 Expression in the Suprachiasmatic Nucleus in Adult Male Rats

Nov 9, 2021Journal of molecular neuroscience : MN

Dopamine D1 Receptors control daily clock gene activity in the brain’s time-keeping center of adult male rats

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Abstract

Dopamine D1 agonist injections significantly altered clock gene expressions and increased nighttime activity in adult male Wistar rats.

  • Dopamine D1 agonist SKF38393 was administered at 20 mg/kg to evaluate its effects on clock gene expressions in the SCN.
  • Injections during the daytime increased Per2 and CLOCK expressions while decreasing Per1 and Bmal1 expressions.
  • At night, SKF38393 significantly increased Per2 expression and decreased Per1, CLOCK, and Bmal1 expressions.
  • Control groups receiving saline showed significantly higher gene expressions overall, except for Per2 during nighttime.
  • SKF injections resulted in a significant increase in running wheel activity during nighttime.

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