Microbial transglutaminase (MTGase) is widely used in food processing, but its potential food safety risks cannot be neglected. MTGase-treated wheat products show immunoreactivity, which may induce celiac disease and damage intestinal barrier function. Therefore, trace detection of MTGase is of great significance. In this study, an electrochemical-colorimetric dual-mode aptasensor for MTGase was developed based on HCR-CRISPR/Cas12a cascade amplification. The system employs high-affinity aptamers for specific recognition of MTGase and significantly enhances the detection signal through HCR-Cas12a cascade amplification, achieving highly sensitive responses. The platform incorporates MXene@AuNPs and Co-Fc-MOF composite materials, where MXene@AuNPs provides excellent conductivity and a stable loading interface, and Co-Fc-MOF offers both electrochemical activity and peroxidase-like activity, enabling synchronous electrochemical and colorimetric signal output. Experimental results demonstrate a wide linear range of 0.5-2000 ng/mL, with a detection limit of 0.12 ng/mL for the electrochemical mode and 0.14 ng/mL for the colorimetric mode. The platform exhibits excellent stability and reproducibility, and achieves satisfactory recovery in real sample analysis. This dual-mode sensing strategy efficiently integrates aptamer recognition, bifunctional nanomaterials, and cascade amplification, providing a rapid and reliable method for trace MTGase detection in food matrices, and offering guidance for the development of multi-mode aptasensor.
