The dysbiosis of gut microbiota attributes to the impairment of blood-brain barrier in rats triggered by cadmium

Oct 11, 2025Toxicology

Gut bacteria imbalance may contribute to blood-brain barrier damage caused by cadmium in rats

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Abstract

Cadmium exposure significantly disturbed gut microbiota and increased blood-brain barrier permeability in rats.

Cadmium consumption was linked to a decrease in short-chain fatty acids in the gut microbiota.
Increased blood-brain barrier permeability was indicated by FITC-dextran leakage and reduced expression of key proteins.
Fecal microbiota transplantation mitigated the effects of cadmium on blood-brain barrier integrity.
Sodium butyrate treatment reduced oxidative stress and improved protein expression levels in endothelial cells affected by cadmium.
Cadmium exposure may contribute to central nervous system health hazards through alterations in gut microbiota.

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Cadmium (Cd) is a non-biodegradable heavy metal with a long biological half-life that is detrimental to human health. As Cd can increase blood-brain barrier (BBB) permeability and disturb the gut microbiota, the relationship between the BBB and gut microbiota disturbance induced by Cd consumption remains unclear. This study aims to identify whether Cd-induced gut microbiota dysbiosis is associated with rat BBB injury and investigate the possible mechanism. Here, we conducted analyses of variations in the composition of the gut microbiota and its metabolites, as well as BBB permeability and the results of the Morris water maze test, in rats treated with Cd by gavage. Fecal microbiota transplantation was performed to verify the role of the microbiota in altering BBB permeability induced by Cd. The results showed that Cd disturbed the gut microbiota, decreasing the levels of short-chain fatty acids (SCFAs). Furthermore, Cd-induced BBB permeability was substantiated by FITC-dextran leakage, ultrastructural observations, and diminished Claudin-5, Occludin, and ZO-1 protein expression, all of which were mitigated by FMT. In vitro, sodium butyrate (SOB) alleviated Cd-induced oxidative stress and increased the expression levels of GPX4 and FTH. Taken together, these findings suggest that Cd disrupts the microbiota and SCFAs components in rats, thereby contributing to BBB damage. SOB prevents Cd-induced BBB damage by suppressing ferroptosis in microvascular endothelial cells. This exhaustive study considerably enhances our comprehension of the health hazards posed by Cd to the central nervous system via the gut-brain axis.

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The dysbiosis of gut microbiota attributes to the impairment of blood-brain barrier in rats triggered by cadmium

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