An ecdysone-responsive nuclear receptor regulates circadian rhythms in Drosophila

Dec 17, 2014Nature communications

A hormone-sensitive receptor controls daily rhythms in fruit flies

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Abstract

Overexpression or knockdown of the nuclear receptor E75 disrupts rest:activity rhythms in Drosophila melanogaster.

  • E75 is associated with circadian functions and was identified through a gain-of-function screen.
  • Manipulating E75 levels in clock neurons dampens molecular oscillations related to circadian rhythms.
  • E75 represses the gene for the transcriptional activator CLOCK (CLK), potentially affecting circadian output.
  • PER may inhibit E75's activity on the Clk promoter, suggesting a regulatory mechanism in circadian transcription.
  • The ecdysone receptor, expressed in central clock cells, produces effects on circadian rhythms similar to E75.
  • E75 appears to help maintain circadian rhythms during stressful conditions, indicating a role for steroid signalling.

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