Effects of Fasting and Re-Feeding on the Expression of Dec1, Per1, and Other Clock-Related Genes

Jul 29, 2006Journal of biochemistry

Fasting and re-feeding change levels of key body clock genes including Dec1 and Per1

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Abstract

Withholding food for 2 days altered the expression of several clock genes in mice.

Dec1 mRNA levels decreased in all tissues examined during fasting.
Per1 mRNA levels significantly increased with fasting.
Per2 expression was reduced in the liver and heart starting 42-46 hours after fasting began.
Dec2 and Bmal1 expression profiles changed in the heart and liver, respectively, during fasting.
Re-feeding after a 36-hour fast partially reversed the effects of fasting on multiple clock genes.
Restriction feeding shifted the phase of expression profiles of all examined clock genes.

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To elucidate the food-entrainable oscillatory mechanism of peripheral clock systems, we examined the effect of fasting on circadian expression of clock genes including Dec1 and Dec2 in mice. Withholding of food for 2 days had these effects: the expression level of Dec1 mRNA decreased in all tissues examined, although Per1 mRNA level markedly increased; Per2 expression was reduced in the liver and heart only 42-46 h after the start of fasting; and expression profiles of Dec2 and Bmal1 were altered only in the heart and in the liver, respectively, whereas Rev-erbalpha mRNA levels did not change significantly. Re-feeding after 36-h starvation erased, at least in part, the effect of fasting on Dec1, Dec2, Per1, Per2, and Bmal1 within several hours, and restriction feeding shifted the phase of expression profiles of all examined clock genes including Dec1 and Dec2. These findings indicate that short-term fasting and re-feeding modulate the circadian rhythms of clock genes to different extents in peripheral tissues, and suggest that the expression of Dec1, Per1, and some other clock genes was closely linked with the metabolic activity of these tissues.

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Effects of Fasting and Re-Feeding on the Expression of Dec1, Per1, and Other Clock-Related Genes

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