Effects of methyl farnesoate on Krüppel homolog 1 (Kr-h1) during vitellogenesis in the Chinese mitten crab (Eriocheir sinensis)

Nov 29, 2020Animal reproduction science

Methyl farnesoate's influence on a key reproductive gene during egg development in the Chinese mitten crab

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Abstract

The Es-Kr-h1 mRNA transcript showed the greatest relative abundance in Stage Ⅱ ovarian development.

Methyl farnesoate (MF) is associated with developmental processes like reproduction and ovarian maturation in crustaceans.
Es-Kr-h1, a gene linked to juvenile hormone signaling, contains seven conserved zinc finger motifs in E. sinensis, but lacks the Zn1 motif.
The addition of MF increased the relative abundance of Es-Kr-h1 mRNA in the hepatopancreas, but not in ovarian tissues.
In vivo experiments indicated that MF treatment and eyestalk removal enhanced Es-Kr-h1 mRNA levels in the hepatopancreas.
The findings suggest that the JH/MF signaling pathway may be conserved between insects and crustaceans.

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Methyl farnesoate (MF), a de-epoxidized form of juvenile hormone (JH) Ⅲ in insects, may regulate developmental processes such as reproduction and ovarian maturation in crustaceans. Krüppel homolog 1 (Kr-h1) is a target response gene for the methoprene-tolerant (Met) protein that is a component of the JH signaling pathway in insects. In the present study, Es-Kr-h1 was cloned from E. sinensis and characterized to ascertain whether JH/MF signaling in insects is conserved in crustaceans. The findings with molecular structure analysis indicated Es-Kr-h1 contains seven zinc finger motifs (Zn2-Zn8) commonly conserved in other crustaceans, but the Zn1 motif was not detected to be present. The PCR results indicated that relative abundance of Es-Kr-h1 mRNA transcript in the hepatopancreas was greatest in the Stage Ⅱ, followed by the Stage Ⅳ ovarian developmental categories. The relative abundance of Es-Kr-h1 mRNA transcript in vitro was greater after MF addition to the hepatopancreas, however, not the ovarian tissues. The results from in vivo and eyestalk ablation experiments indicated the relative abundance of Es-Kr-h1 mRNA transcript was greater after MF treatment and bilateral eyestalk removal in the hepatopancreas, however, not ovarian tissues. Notably, there were effects of MF on relative abundance of Es-Kr-h1 mRNA transcript pattern. The Es-Kr-h1 protein, therefore, may be involved in MF-mediated vitellogenesis resulting from the response to Es-Met in E. sinensis, and the JH/MF signaling pathway is potentially conserved in crustaceans.

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Effects of methyl farnesoate on Krüppel homolog 1 (Kr-h1) during vitellogenesis in the Chinese mitten crab (Eriocheir sinensis)

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