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Potential role of Methoprene-tolerant (Met) in methyl farnesoate-mediated vitellogenesis in the Chinese mitten crab (Eriocheir sinensis)
Possible role of Methoprene-tolerant in hormone-driven egg development in the Chinese mitten crab
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Abstract
The methyl farnesoate (MF) titer was significantly increased in late exogenous vitellogenic oocytes (stage III).
- Methoprene-tolerant (Met) is a candidate receptor for juvenile hormone in insects and is also involved in crustacean reproduction.
- The full-length cDNA for Met was cloned from the Chinese mitten crab (Eriocheir sinensis), revealing three conserved domains associated with hormone binding.
- EsMet mRNA is highly expressed in the hepatopancreas, particularly during ovarian development stages.
- In vitro, MF addition significantly increased EsMet and EsVg expression in hepatopancreatic tissue.
- In vivo studies showed that MF injection synchronously raised EsMet and EsVg expression in the hepatopancreas, but not in the ovaries.
- Eyestalk ablation resulted in increased expression of EsMet and EsVg in the hepatopancreas, with EsVg also induced in the ovaries.
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