The New phytologistJul 28, 2026

Precise replacement and removal of large DNA sections in rice using template-jumping prime editing

CRISPR Gene Editing Weekly Brief ↗PubMed ↗DOI ↗

Updated Aug 3, 2026

Abstract

Template-jumping prime editing (TJ-PE) enables the replacement of genomic fragments in rice of up to 340 bp and precise deletions of 944 to 2024 bp.

TJ-PE can replace genomic sequences with homologous DNA fragments ranging from dozens to hundreds of base pairs.
The method achieved precise deletion efficiencies of up to 34.6% for approximately 2000-bp segments.
This approach enhances the potential for large-scale genomic editing in rice.
TJ-PE establishes a versatile tool for both deletion and replacement of significant DNA segments.

Simplified

Homologous replacement of genomic sequences with large DNA fragments (> 100 bp) holds great potential for crop breeding, yet an efficient method to achieve such edits is lacking in plants. Here, in rice, we developed template-jumping prime editing (TJ-PE), a recently reported PE strategy for large targeted insertion, as an efficient tool for homologous replacement with DNA fragments ranging from dozens to hundreds of base pairs, and using TJ-PE, we replaced genomic fragments of up to 340 bp with homologous fragments of the same length. In addition, our TJ-PE tool also enabled precise deletion of 944- to 2024-bp fragments in rice, with efficiencies of up to 34.6% for c. 2000-bp precise deletions. Collectively, this study expands the editing scope of PE in rice and establishes TJ-PE as a generalist tool for precise deletion and replacement of large DNA fragments.

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Precise replacement and removal of large DNA sections in rice using template-jumping prime editing

Abstract

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