Efficient production of a self-copying RNA vaccine using a single enzyme from Klebsiella phage 32 starting with its natural RNA code

KP32 RNA polymerase demonstrates a 9.3-fold improvement in production levels of self-amplifying RNA compared to T7 RNA polymerase.

• KP32 RNA polymerase efficiently produces RNA of varying lengths.
• It shows a wide tolerance range for temperature, pH, and salt.
• The enzyme is capable of producing an 11,551 nucleotide self-amplifying RNA.
• The native initiating dinucleotide, AU, is critical for its RNA production.

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