Gene Editing Using Electric Pulse Delivery of Cas9 Protein and Modified Guide RNAs

Electroporation-based delivery of Cas9 protein achieves high efficiency in difficult-to-transfect human cancer cell lines and primary cells.

• CRISPR/Cas9 is a widely used tool for genome editing in many human cancer cell lines.
• Gene editing remains challenging in certain hard-to-transfect cell lines and primary cells.
• An electroporation-based method has been developed to improve the delivery of Cas9 protein.
• This method utilizes chemically modified sgRNAs for enhanced performance.
• The protocol demonstrates significant efficiency across various cell lines and primary cells.

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