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Abstract
Engineered pegRNAs (epegRNAs) improve prime editing efficiency by 3-4 times in various human cell types.
- Prime editing allows for precise modifications in DNA, including point mutations and small insertions or deletions.
- Degradation of specific regions of the pegRNA can diminish the effectiveness of prime editing.
- Incorporating structured RNA motifs at the 3' end of pegRNAs enhances their stability and editing efficiency.
- The modified pegRNAs maintain a low risk of off-target effects during DNA editing.
- Optimized structural motifs and a new computational tool (pegLIT) were developed to improve pegRNA design.
- epegRNAs facilitate the installation or correction of mutations associated with diseases.
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