Enhanced binding interaction and antibacterial inhibition for nanometal oxide particles activated with Aloe Vulgarize through one-pot ultrasonication techniques

Jun 21, 2024Bioorganic chemistry

Improved antibacterial effects of nanometal oxide particles activated with Aloe Vera using a one-step ultrasound method

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Abstract

The minimum inhibitory concentration (MIC) for all tested bacterial strains is at 3.125 µg/ml.

Green zinc oxide nanoparticles (ZnO NPs) synthesized from Aloe Vera leaf peel extract exhibit a flower-like structure that may enhance bacterial inhibition.
The antibacterial effect of ZnO NPs is stronger when combined with active ingredients from Aloe Vera, shown by larger zones of inhibition.
Molecular docking studies indicate that low binding energy values suggest significant interaction between Aloe Vera components and selected protein targets.
Cytotoxicity tests show that ZnO NPs at concentrations below 20 µg/ml are nontoxic to human dermal fibroblast (HDF) cells.
Probe sonication of ZnO NPs with 20% Aloe Vera is identified as an efficient synthesis method with effective antibacterial properties.

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The interaction of green zinc oxide nanoparticles (ZnO NPs) with bacterial strains are still scarcely reported. This work was conducted to study the green-one-pot-synthesized ZnO NPs from the Aloe Vulgarize (AV) leaf peel extract assisted with different sonication techniques followed by the physicochemical, biological activities and molecular docking studies. The NPs structure was analyzed using FTIR, UV-vis and EDX. The morphology, particle size and crystallinity of ZnO NPs were identified using FESEM and XRD. It was found that the formed flower-like structure with sharp edge and fine size of particulates in ZnO NPs/AV could enhance the bacterial inhibition. The minimum inhibitory concentration (MIC) for all the tested bacterial strains is at 3.125 µg/ml and the bacterial growth curve are dependent on the ZnO NPs dosage. The results of disc diffusion revealed that the ZnO NPs/AV possess better antibacterial effect with bigger ZOI due to the presence of AV active ingredient. The molecular docking between active ingredients of AV in the NPs with the protein of IFCM and 1MWU revealed that low binding energy (E = -6.56 kcal/mol and -8.99 kcal/mol, respectively) attributes to the excessive hydrogen bond from AV that highly influenced their interaction with the amino acid of the selected proteins. Finally, the cytotoxicity test on the biosynthesized ZnO NPs with concentration below 20 µg/ml are found nontoxic on the HDF cell. Overall, ZnO NPs/20 % AV (probe sonication) is considered as the best synthesis option due to its efficient one-pot method, short sonication time but own the best antibacterial effect. bind

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Enhanced binding interaction and antibacterial inhibition for nanometal oxide particles activated with Aloe Vulgarize through one-pot ultrasonication techniques

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