Enzymatic Synthesis of Psilocybin

Four enzymes responsible for psilocybin biosynthesis were characterized.

• PsiD is identified as a new type of enzyme that converts l-tryptophan into another compound.
• PsiK facilitates the transfer of a phosphate group during the biosynthesis process.
• PsiM is responsible for adding methyl groups as the final step in creating psilocybin.
• PsiH acts as a monooxygenase, playing a role in the biosynthetic pathway.
• An enzymatic synthesis of psilocybin was achieved from 4-hydroxy-l-tryptophan using PsiD, PsiK, and PsiM.

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