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Rapid one-step detection of food bacteria using enzyme-driven CRISPR/Cas12a without signal amplification or PAM requirement
Updated
Abstract
Essence
A restriction-enzyme CRISPR/Cas12a strategy enabled one-pot bacterial DNA detection without PAM dependence or amplification.
Evidence
This molecular detection platform experiment tested food-contaminating bacteria detection for Alicyclobacillus acidoterrestris and Cronobacter sakazakii, reaching 13.38 CFU/mL and 18.67 CFU/mL sensitivity within 60 minutes.
Caveat
The work validates an assay strategy for selected bacteria, not real-world clinical or food-safety deployment across diverse samples.
Simplified
CRISPR Gene Editing