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Identification of ETH receptor and its possible roles in the mud crab Scylla paramamosain
Finding the ETH receptor and its possible functions in the mud crab
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Abstract
A 16-day administration of Ecdysis-triggering hormone (SpETH) significantly increased gonadosomatic index and oocyte size in the mud crab Scylla paramamosain.
- A G protein-coupled receptor for SpETH (SpETHR) was identified in S. paramamosain and activated by SpETH with an EC value of 75.18 nM.
- SpETH was found in the cerebral and thoracic ganglia, while SpETHR was specifically expressed in the ovary, hepatopancreas, and Y-organ of female crabs.
- In vitro, synthetic SpETH at 10 µM increased the expression of the vitellogenin receptor (SpVgR) in the ovary and induced ecdysone production in the Y-organ.
- In vivo, SpETH injection led to significant upregulation of ecdysone receptor (SpEcR) in the ovary and other developmental factors in the Y-organ.
- SpETH administration is associated with increased 20-hydroxyecdysone levels in hemolymph, indicating potential involvement in ovarian development.
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