The Evening Complex Establishes Repressive Chromatin Domains Via H2A.Z Deposition

Nov 13, 2019Plant physiology

The Evening Complex Helps Create Gene-Silencing Regions by Adding H2A.Z Protein

AI simplified

Circadian Biology on OpenScience ↗PubMed ↗DOI ↗

Abstract

The Evening Complex (EC) in Arabidopsis regulates gene expression by inducing repressive chromatin structure at dusk.

The EC integrates temperature information with environmental signals to influence gene expression.
ELF3, a component of the EC, interacts directly with a protein from the SWR1 complex to manage H2A.Z-nucleosome deposition.
SWR1 components exhibit circadian oscillation in gene expression, peaking at dusk.
Disruption in SWR1 activity alters the expression of morning-expressed genes, indicating its role in the circadian clock.
The EC-SWR1 complex binds to key clock gene loci and facilitates nucleosome deposition, aligning with gene repression at dusk.

AI simplified

The Evening Complex (EC) is a core component of the Arabidopsis () circadian clock, which represses target gene expression at the end of the day and integrates temperature information to coordinate environmental and endogenous signals. Here we show that the EC induces repressive chromatin structure to regulate the evening transcriptome. The EC component ELF3 directly interacts with a protein from the SWI2/SNF2-RELATED (SWR1) complex to control deposition of H2A.Z-nucleosomes at the EC target genes. SWR1 components display circadian oscillation in gene expression with a peak at dusk. In turn, SWR1 is required for the circadian clockwork, as defects in SWR1 activity alter morning-expressed genes. The EC-SWR1 complex binds to the loci of the core clock genes-() andand catalyzes deposition of nucleosomes containing the histone variant H2A.Z coincident with the repression of these genes at dusk. This provides a mechanism by which the circadian clock temporally establishes repressive chromatin domains to shape oscillatory gene expression around dusk. Arabidopsis thaliana PSEUDO RESPONSE REGULATOR7PRR7PRR9

Full Text

Full text is available at the source.

View full text ↗

The Evening Complex Establishes Repressive Chromatin Domains Via H2A.Z Deposition

Abstract

Full Text

You found one interesting study. We’ll send the next 7.

what lands in your inbox each week:

Recent issues from the circadian biology brief

Abstract

Full Text

Related papers

You found one interesting study. We’ll send the next 7.

what lands in your inbox each week:

Recent issues from the circadian biology brief