Using engineered virus-like particles to deliver gene editing tools in pancreatic cancer cells

Pancreatic ductal adenocarcinoma (PDAC) cell lines may be transfected using engineered virus-like particles (eVLPs) for gene editing.

• PDAC cell lines have inherent resistance to traditional transfection methods.
• CRISPR-Cas9 and prime editing can be delivered into PDAC cells using eVLPs.
• The protocol allows for the packaging of CRISPR components with engineered guide RNAs.
• Successful gene edits were demonstrated at the HEK3 locus and NQO1 exon 6.
• This method can be adapted for targeting other genomic locations.

Simplified