Full text is available at the source.
Journal of ethnopharmacology···
Gut bacteria and metabolism changes in rats with kidney-yang deficiency and the effects of You-gui pill
Updated
Abstract
KYDS is associated with 21 differential metabolites and seven potential metabolic pathways.
- KYDS is linked to metabolic disorders involving amino acid metabolism, energy metabolism, and short-chain fatty acid metabolism.
- Eleven distinct intestinal microbiota species are associated with KYDS, influencing various metabolic processes.
- Three gut microbiota (Bacteroides, Desulfovibrio, and [Eubacterium]_coprostanoligenes_group) are primarily connected to KYDS-related metabolic disturbances.
- YGP mediates the activity of four specific microbes and ten metabolites, improving metabolic disorders in KYDS.
- The findings suggest a close relationship between KYDS, microbial dysbiosis, and metabolic dysfunction.
Simplified
ETHNOPHARMACOLOGICAL RELEVANCE: A myriad of evidence have shown that kidney-yang deficiency syndrome (KYDS) is associated with metabolic disorders of the intestinal microbiota, while TCMs can treat KYDS by regulating gut microbiota metabolism. However, the specific interplay between KYDS and intestinal microbiota, and the intrinsic regulation mechanism of You-gui pill (YGP) on KYDS' gut microbiota remains largely unknown so far.
MATERIALS AND METHODS: In the present study, fecal metabonomics combined with 16S rRNA gene sequencing analysis were used to explore the mutual effect between KYDS and intestinal flora, and the intrinsic regulation mechanism of YGP on KYDS's gut microbiota. Rats' feces from control (CON) group, KYDS group and YGP group were collected, and metabolomic analysis was performed usingH NMR technique combined with multivariate statistical analysis to obtain differential metabolites. Simultaneously, 16S rRNA gene sequencing analysis based on the Illumina HiSeq sequencing platform and ANOVA analysis were used to analyze the composition of the intestinal microbiota in the stool samples and to screen for the significant altered microbiota at the genus level. After that, MetaboAnalyst database and PICRUSt software were apply to conduct metabolic pathway analysis and functional prediction analysis of the screened differential metabolites and intestinal microbiota, respectively. What's more, Pearson correlation analysis was performed on these differential metabolites and gut microbiota. 1
RESULTS: Using fecal metabonomics, KYDS was found to be associated with 21 differential metabolites and seven potential metabolic pathways. These metabolites and metabolic pathways were mainly involved in amino acid metabolism, energy metabolism, methylamine metabolism, bile acid metabolism and urea cycle, and short-chain fatty acid metabolism. Through 16S rRNA gene sequencing analysis, we found that KYDS was related to eleven different intestinal microbiotas. These gut microbiota were mostly involved in amino acid metabolism, energy metabolism, nervous, endocrine, immune and digestive system, lipid metabolism, and carbohydrate metabolism. Combined fecal metabonomics and 16S rRNA gene sequencing analysis, we further discovered that KYDS was primarily linked to three gut microbiotas (i.e. Bacteroides, Desulfovibrio and [Eubacterium]_coprostanoligenes_group) and eleven related metabolites (i.e. deoxycholate, n-butyrate, valine, isoleucine, acetate, taurine, glycine, α-gluconse, β-glucose, glycerol and tryptophan) mediated various metabolic disorders (amino acid metabolism, energy metabolism, especially methylamine metabolism, bile acid metabolism and urea cycle, short-chain fatty acid metabolism. nervous, endocrine, immune and digestive system, lipid metabolism, and carbohydrate metabolism). YGP, however, had the ability to mediate four kinds of microbes (i.e. Ruminiclostridium_9, Ruminococcaceae_UCG-007, Ruminococcaceae_UCG-010, and uncultured_bacterium_f_Bacteroidales_S24-7_group) and ten related metabolites (i.e. deoxycholate, valine, isoleucine, alanine, citrulline, acetate, DMA, TMA, phenylalanine and tryptophan) mediated amino acid metabolism, especially methylamine metabolism, bile acid metabolism and urea cycle, short-chain fatty acid metabolism, endocrine, immune and digestive system, and lipid metabolism, thereby exerting a therapeutic effect on KYDS rats.
CONCLUSION: Overall, our findings have preliminary confirmed that KYDS is closely related to metabolic and microbial dysbiosis, whereas YGP can improve the metabolic disorder of KYDS by acting on intestinal microbiota. Meanwhile, this will lay the foundation for the further KYDS's metagenomic research and the use of intestinal microbiotas as drug targets to treat KYDS.
Related papers
Jul '19
How the You-Gui Pill May Affect Body Systems and Metabolism in Treating Kidney-Yang Deficiency
top 20% journal
cited by 22 papers
journal article
May '22
Biomarkers and treatment effects of kidney-yang deficiency syndrome using You-gui pill analyzed by system-level drug action and blood metabolism
top 30% journal
cited by 23 papers
journal article
May '22
Gut and feces analysis show Gushudan may protect kidney function in rats with kidney-yang deficiency
top 30% journal
cited by 19 papers
journal article
Jan '24
Effects of Epimedium Before and After Fat Processing on Gut Bacteria and Metabolism in Rats with Kidney Yang Deficiency
top 30% journal
cited by 3 papers
journal article
May '21
Gut bacteria and feces chemicals show kidney protection by Rehmanniae Radix Preparata and Corni Fructus in rats with kidney disease caused by adenine
top 50% journal
cited by 39 papers
journal article
Sep '20
Baoyuan decoction's heart protection linked to gut metabolism
top 10% journal
cited by 38 papers
journal article
Nov '19
Scutellariae root and coptis rhizome improve sugar and fat metabolism in type 2 diabetic rats by changing gut bacteria and their products
top 20% journal
cited by 155 papers
journal article
Feb '24
Metabolite and gut microbe changes linked to Gushudan preventing kidney-yang deficiency in rats
top 30% journal
cited by 9 papers
journal article
Jul '19
Yin-Chen-Hao Tang's protective effects against sudden liver damage caused by carbon tetrachloride using gut bacteria and metabolism analysis
top 30% journal
cited by 39 papers
journal article
Sep '17
Using Chinmedomics to Identify Active Ingredients and How Nanshi Capsule Works for Kidney-Yang Deficiency Syndrome
cited by 1 paper
journal article