BMC methods

An Easy Fluorescent Method to Improve Gene Editing in Human Stem Cells

Updated

Abstract

Prime editing minimizes genomic instability and off-target effects by avoiding double-stranded DNA breaks.

  • This technique allows for precise insertions, deletions, and substitutions in the genome.
  • Prime editing surpasses the limitations of traditional CRISPR-based genome-editing methods.
  • Low editing efficiency in human pluripotent stem cells may limit its use in stem cell research and regenerative medicine.

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Competing interests

0 of 8
authors report competing interests
8 report none
PubMed

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