Genomic tests to identify interactions between gene control regions and gene starters across the genome

Recent advances in high-throughput methods have enhanced understanding of enhancer-promoter interactions.

• Enhancers play a crucial role in regulating gene transcription at promoters.
• Chromosome conformation capture assays, like Hi-C, help study chromatin architecture.
• Ectopic reporter experiments, such as STARR-seq, quantify promoter and enhancer activity.
• Endogenous perturbations using CRISPRi identify compatibility between enhancers and promoters.
• These methods contribute to a deeper understanding of gene regulatory networks.

Simplified