Gene Editing Using Combined Guide RNAs for Cas9 and Cas12a

Fusion guide RNAs (fgRNAs) may enable multiplex genome editing in human cells.

• The CRISPR-Cas system uses Cas proteins and guide RNA complexes to target and cut specific DNA sequences.
• Cas9 and Cas12a are two types of endonucleases involved in genome editing.
• Engineering of both Cas proteins and guide RNAs has expanded the potential uses of the CRISPR-Cas system.
• The development of fgRNAs allows for orthogonal gene manipulation, which could enhance gene editing capabilities.

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