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Gene Manipulation Using Fusion Guide RNAs for Cas9 and Cas12a
Gene Editing Using Combined Guide RNAs for Cas9 and Cas12a
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Abstract
Fusion guide RNAs (fgRNAs) may enable multiplex genome editing in human cells.
- The CRISPR-Cas system uses Cas proteins and guide RNA complexes to target and cut specific DNA sequences.
- Cas9 and Cas12a are two types of endonucleases involved in genome editing.
- Engineering of both Cas proteins and guide RNAs has expanded the potential uses of the CRISPR-Cas system.
- The development of fgRNAs allows for orthogonal gene manipulation, which could enhance gene editing capabilities.
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