Journal of visualized experiments : JoVE

Using Genetic Deletion of Gene Control Regions to Study Non-Coding DNA Function in Early Human Development Models

CRISPR Gene Editing Weekly Brief ↗PubMed ↗DOI ↗

Updated

Abstract

A substantial proportion of transcriptionally active cis-regulatory elements in primate embryos originated from ancient retroviral integrations into the germline.

  • Endogenous retroviruses may retain intrinsic regulatory activity and could influence species-specific aspects of embryonic development.
  • Ethical restrictions on human embryo research have limited direct studies of gene regulation during human embryogenesis.
  • Human naive pluripotent stem cells and three-dimensional blastocyst models offer alternative systems for investigating early developmental processes.
  • CRISPR-Cas9 technology can be used to delete retrovirus-derived cis-regulatory elements in human naive pluripotent stem cells.
  • The method allows for the creation of various deletion clones to study the role of individual retrovirus-derived elements in gene regulation.

Simplified

Full Text

Full text is available at the source.
View Full Text ↗

Related papers

Jun '22
TEAD4 controls early outer cell development before CDX2 without relying on GATA3 in human embryos
cited by 29 papers
research support, non-u.s. gov't
Apr '16
Using Gene Editing to Delete One Copy of DNA Control Regions in Mouse Embryonic Stem Cells to Study Their Function
top 50% journal
cited by 14 papers
research support, non-u.s. gov't

What Lands in Your Inbox Each Week:

  • 📚7 fresh studies
  • 📝plain-language summaries
  • ✅direct links to original studies
  • 🏅top journal indicators
  • 📅weekly delivery
  • 🧘‍♂️always free

Recent issues from the crispr gene editing brief

issue #52
CRISPR-edited red blood cells engineered to trap HIV before it reaches its targets
2 weeks ago7 studies
issue #51
Virus-like particles just unlocked CRISPR screening in immune cells that were nearly impossible to edit
3 weeks ago7 studies
issue #50
A machine learning model just made prime editing dramatically easier to design
4 weeks ago7 studies
issue #49
A base editor corrected a dangerous immune mutation with 90% efficiency in patient stem cells
5 weeks ago7 studies
issue #48
CRISPR base editors delivered to rat brains reduced Huntington's protein fragments and improved movement
6 weeks ago7 studies
issue #47
AI tool uses AlphaFold3 to pinpoint exactly which gene editor residues cause off-target cuts
7 weeks ago7 studies
issue #46
A new delivery trick pushed prime editing efficiency up to 2.9-fold without extra off-target edits
8 weeks ago7 studies
issue #45
Scientists built humanized antibody mice in 8 weeks instead of over a year
9 weeks ago7 studies
issue #44
Scientists mapped 11,692 gene knockdowns in stem cells — all in one atlas
10 weeks ago7 studies
issue #43
A rat virus enzyme boosted gene editing efficiency by 1.88x at hard-to-edit DNA sites
11 weeks ago7 studies
explore the archive
See more issues from the crispr gene editing brief