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Abstract
Recent advances enable programmable manipulation of DNA fragments ranging from kilobases to megabases in plants.
- Technologies such as double-strand break, prime-editing, recombinase, and transposase facilitate various genome modifications.
- These modifications include deletion, insertion, replacement, duplication, inversion, and translocation of genetic material.
- The review compares the strengths and limitations of major strategies for large-fragment genome engineering.
- Applications of these technologies extend to fundamental plant biology and crop breeding.
- Integration of artificial intelligence is enhancing the optimization of large-fragment genome engineering.
- The shift from genome editing to chromosome engineering may significantly impact crop breeding and plant synthetic biology.
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